Immunoregulatory effects of human amniotic mesenchymal stem cells and their exosomes on human peripheral blood mononuclear cells

Background: The immunomodulatory effects of mesenchymal stem cells (MSCs) and their exosomes have been receiving increasing attention. This study investigated the immunoregulatory human amniotic (hAMSCs) on phytohemagglutinin (PHA)-induced peripheral blood mononuclear (PBMCs). Methods: hAMSCs used in experiment were identified by light microscopy flow cytometry, differentiation ability was determined Oil Red O Alizarin staining. expressions transforming growth factor (TGF)-β, indoleamine 2,3-dioxygenase (IDO), cyclooxygenase-2 (COX-2), hepatocyte (HGF), interleukin (IL)-6 detected quantitative real-time polymerase chain reaction western blotting. PBMCs, hAMSCs, collected for in vitro group culture. Then analyzed cytometry Enzyme-linked immunosorbent assay. Results: successfully extracted from membrane. TGF-β, IDO, COX-2, HGF, IL-6 significantly expressed hAMSCs. In co-culture showed that promoted proliferation Th2 PHA-induced while inhibited secretion TNF-α IL-4 IL-10, had more significant than exosomes. Conclusions: or could exert PBMCs affecting cell cytokine secretion.